Design and Characterization of a Zn2+-Binding Four-Helix Bundle Protein in the Biophysical Chemistry Laboratory
نویسندگان
چکیده
منابع مشابه
The design of a four-helix bundle protein.
The design of proteins with predetermined structural properties is a necessary first step in the de novo design of novel enzymes and receptors. A major problem associated with the design of proteins is the high flexibility inherent in polypeptide chains. It has been estimated that a protein of 100 residues can adopt up to 101~176 different conformations, a number that is as large as the number ...
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Rationally redesigned variants of the 4-helix-bundle protein Rop are described. The novel proteins have simplified, repacked, hydrophobic cores and yet reproduce the structure and native-like physical properties of the wild-type protein. The repacked proteins have been characterized thermodynamically and their equilibrium and kinetic thermal and chemical unfolding properties are compared with t...
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The Biophysical Chemistry Laboratory (BCL) was established in 1986 at Institute of Biochemistry and Biophysics (IBB), University of Tehran as the main base and the mother of biophysical chemistry in Iran. BCL is a famous worldwide research lab in the area of thermodynamics of protein denaturation and biomacromolecular interaction. The first student that joined BCL was MSc student in 1987, while...
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The protein Felix was designed de novo to fold into an antiparallel four-helix bundle of specific topology. Its sequence of 79 amino acid residues is not homologous to any known protein sequence, but is "native-like" in that it is nonrepetitive and contains 19 of the 20 naturally occurring amino acids. Felix has been expressed from a synthetic gene cloned in Escherichia coli, and the protein ha...
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A molecular understanding of volatile general anesthetic mechanisms of action will ultimately require high-resolution structural descriptions of anesthetic–protein complexes. Structural changes in proteins following anesthetic binding have been technically difficult to detect, but presumably underlie many of the resulting reversible alterations in protein function. Using three different spectro...
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ژورنال
عنوان ژورنال: Journal of Chemical Education
سال: 2014
ISSN: 0021-9584,1938-1328
DOI: 10.1021/ed400368c